Optically transparent single crystal; L-Alanine doped EDTA is fruitfully grown using slow evaporation technique at room temperature. The grown crystals have been characterized morphologically, analyzed spectroscopically and thereby crystal and structural properties have been interpreted. The crystal parameters have been calculated from experimentally observed data and the calculated values proved orthorhombic crystal lattice formation. The variable refractive indices for different coordinates were measured and it ensured birefringence effect in the crystal. Thermal properties were studied from the data observed from TGA and DSC instruments and thermal stability of the sample was validated. The structural and morphological influence of LAlanine on EDTA was analyzed by SEM.