Aim: The aim of the research work is to improve the entropy of Red Green Blue (RGB) image encryption using novel Deoxy Nucleic Acid (DNA) Exclusive OR encoding in comparison with DNA additive encoding using MATLAB software. Materials and Methods: The image encryption is performed using novel DNA Exclusive OR encoding with number of samples (N=10) and additive encoding with number of samples (N=10) having pre-test power of 80% using MATLAB software. Results: The entropy of Novel DNA Exclusive OR encoding is 1.62056 whereas the entropy of DNA additive encoding is 1.53383. There is a significant difference in the results obtained for entropy and were considered to be error-free since it has the significance value p = 0.0329 (p<0.5) in SPSS statistical analysis. Conclusion: Novel DNA Exclusive OR encoding performs significantly better in increasing entropy in RGB image encryption when compared to DNA additive encoding