Due to their wide-ranging applicability in numerous fields, nanoparticles (NPs) are in profitable demand. Traditional chemical synthesis procedures are employed to create metallic nanoparticles, and the chemicals utilised are frequently hazardous, volatile, and expensive. The development of dependable, long-lasting, and environmentally acceptable methods for creating metallic nanoparticles is now a key advancement in the field of nanotechnology. Plant extracts can replace the expensive and inefficient large-scale manufacturing of nanoparticles since they are environmentally benign and cost-effective. In the current study, we developed stable Titanium oxide nanoparticles utilising a unique method that involved using Cynodon dactylon leaf extract and experiencing a sudden decline in titanium ions.The titanium element is present in significant amounts in TiO2 NPs, which is equivalent to a high oxygen concentration. Using the FT-IR spectrum, the functional group and chemical component contained in the produced TiO2 NPs were located. The stretching and bending vibration of the -OH group is what causes the peaks at 3189.08 and 1634.22 cm-1 in the spectrum. The monodisperse character of the particles, with an average particle size of about 524 dnm, is revealed by the DLS results. Zeta potential was used to assess the stability of nanoparticles. It was discovered that the zeta potential value was strongly negative, or 31.7 mV, which suggests that the particles were well colloidal and steadily stable. The TiO2 nanoparticles' TGA-DTA curve exhibits a progressive decline with increasing temperature under inert conditions.