A needle stick injury are wounds caused by needles that are accidently puncture the skin. These injuries can occur at any time when people use disassemble or dispose of needle. To assess the pre-test and post-test level of knowledge and prevention of needle stick injuries among nursing student. The research approach was quantitative research design adopted experimental design. The sample was selected by purposive sampling technique. The sample size for the study was71.The study findings revealed that the pre-test, 45(63.38%) had inadequate knowledge, 23(32.39%) had moderate adequate knowledge and 3(4.23%) had adequate knowledge. The post test, 39(54.93%) had moderate adequate knowledge, 26(36.62%) had adequate knowledge and 6(8.45%) had inadequate knowledge and prevention of needle stick injuries. This study finding shows that demographic variables occupation of father and residential area had shown statistically significant association with post-test level of knowledge and prevention of needle stick injury among nursing students at p<0.05 level. From the analysis outcome found that there was moderate applicable understanding about knowledge and prevention of needle stick injury among nursing students. After conducting structured teaching on needle stick injury helps them in understanding to maintain adequate knowledge on needle stick injury.