Introduction: Nanotechnology is a rapidly manifesting scientific field with various biomedical and pharmaceutical applications. Among the various metal nanoparticles, silver has gained popularity because of its potent antibacterial effects. In this investigation, silver nanoparticles were synthesized by bromelain, which is an elementary extract from pineapple employing a nontoxic, cost efficient and environmental friendly process. The aim of the present study was to synthesize bromelain mediated silver nanoparticles and to evaluate its antibacterial efficacy against E-faecalis. Materials and Methods: Aqueous extract of bromelain was formulated. An ecofriendly green synthesis was employed to fabricate bromelain mediated silver nanoparticles. The synthesized nanoparticles were characterized by UV- visible absorption spectra. The structure of the nanoparticles was analyzed by TEM. The antibacterial activity of silver nanoparticles was determined by agar well diffusion method against E.faecalis. Results: Antibacterial activity demonstrates that the zone of inhibition of E-faecalis varies with an increase in concentration of Ag and remains constant at 50μl and 100μl respectively. The characterization of Ag+ ions by UV- visible absorption spectra confirms the reduction of silver ions to silver nanoparticles. TEM affirms that the synthesized nanoparticles were of spherical shape and the size of the nanoparticles ranges from 3-20nm. Conclusion: This study states that bromelain mediated silver nanoparticles possess good antibacterial properties.