Assessment of soil organic carbon (SOC) in terrestrial ecosystems, particularly in tropical deciduous forests is one of the global imperative tasks in order to better comprehend its contribution in regulating the regional and global carbon cycles. Yok Don National Park is dominated by deciduous forest with smaller areas of evergreen forest, particularly on hills and along watercourses. The objectives of this study were to investigate the impacts of various environmental factors on the density of soil organic carbon (SOC) in the deciduous forest areas of Yokdon National Park and analyzed the effects of soil type, components in soil, forest status, steepness, altitude on %OC as well as estimation of total SOC stock using the Normalized Difference Vegetation Index (NVDI) and Factor Analysis for Mixed Data (FAMD). The results showed that soil type and soil texture had the most significant impacts on %OC, accounting for over 50% of the variation in OC% and closely linked to changes in %OC. The total storage of SOC in the dipterocarp forest of Yok Don National Park is estimated to be approximately 7,644,080 tons across an area of 95,955.23 ha. The SOC stock shows a large fluctuation range from 14.3 tons/ha to 246.8 tons/ha with an average of 84 tons/ha, respectively. The effective protection and management practices implemented in Yok Don National Park have played a significant role in enhancing SOC storage in the forest soils of this region. Our findings in this study may provide useful information for forest conservation and management as pertaining to strategies for the preservation and augmentation of carbon storage within forest ecosystems.