Objectives: DermaLab Combo is an advanced multiparameter skin analysis system developed by Cortex Technology. Though the reliability of the instrument using test-retest and inter-rater reliability was analyzed in previous studies, no studies compared the measurement’s accuracy histologically. The study aimed to compare the skin thickness of animal skin measured by DermaLab Combo to the skin thickness measurements obtained from the frozen section and permanent section. Materials and Methods: This study included 4 healthy male or female (totally 8 sites) rodents weighing at least 160 grams. The outcome assessed was skin thickness. After skin thickness was measured with the ultrasound (USG) probe of DermaLab Combo, an incisional biopsy of the site was done and sent for the frozen section and permanent paraffin section for histological analysis of skin thickness. The data were tabulated and analyzed with SPSS software. Results: There is no significant difference in the measurement of skin thickness by the USG probe of DermaLab Combo and histological skin thickness assessed by the gold standard permanent paraffin sectioning (p=0.575>0.05). Conclusion: We conclude that the USG probe of DermaLab Combo is reliable in measuring skin thickness for clinical and research purposes.