A pilot study was conducted to determine the efficacy of fractional CO2 laser intravaginally for the management of mixed urine incontinence (MUI). Materials and methods: This is a prospective, experimental study. An overall of 13 patients with confirmed urodynamic MUI were included. All patients received two sessions of FRAXIS fractional CO2 laser, model FRX-C1 separated by 4 weeks. Three months after second laser therapy, Patients were requested to come in for a clinical evaluation by urodynamics. Also, adverse events were recorded. Results: Approximately two thirds of patients reported variant degrees of improvement up to 3 months after the second laser session [5 (38.5%) patients showed improvement od MUI symptoms and 3 patients stated that they have no more incontinence (23.1%)]. Furthermore, urodynamics study showed that 3 patients had no more SUI by Abdominal Leak Point Pressure (ALPP) and 2 patients had no Detrusor Overactivity (DO). During the course of the study, there were no major adverse events. Conclusions: the use of Transvaginal fractional CO2 laser may be an effective treatment for MUI. It is expected to be a less invasive, safe, and quicker-recovery alternative to traditional management.