The study aimed to establish the baseline data of Bisphenol A (BPA) presence in the unexplored, and the largest district named Kachchh. BPA is a monomer and endocrine disruptor that is utilized in plastic and epoxy resin manufacture. The fact that it is widely used, and its possible health threats call for the urgent monitoring of BPA in environmental as well as human samples. The study adopted analytical method to detect baseline level of BPA leaching, due to temperature variations in mineral water bottles and pouches, as well as urine samples from gynecological patients. A Waters HPLC device, equipped with a 515 pump and an ultraviolet detector at 278 nm, was used to detect BPA in a methanol-water mobile phase using the 2489 detector. Findings suggested that BPA concentration in stored pouches were significantly higher in comparison to the samples taken directly from the refrigerator. Also, the BPA level was almost (0.25-2.1 µg/L) in the urine sample of the Gynec patient compared with the standard peaks. The results prove the urgent need for more awareness about BPA contamination across this region. Such contamination may have the effect of environmental pollution and thus affects public health. The determinations of high BPA levels in the stored water containers for drinking purposes aroused the issues of long-term exposure to this hormone disruptor chemical. Also, sensitive method of detection to be performed in the future as extended part of this study. Consequently, it is essential that regulations and consumer awareness education are implemented to minimize the BPA exposure risks and protect human health as well as the environment. 8448108108