The in-situ hydrogel drug delivery system has attracted attention from more and more researchers in recent years. In the present research work effect of gellan gum, CaCO3 and HPMC on water uptake time, gelation time as well as floating lag time of in-situ hydrogel preparation of nizatidine was studied. Various concentration of gellan gum, HPMC and CaCO3 were used in different formulations. Data showed that both the gelation time and the floating-lag time could be minimized through increased amount of HPMC. It has been discovered that the incorporation of divalent (Ca2+) ions into gellan gels results in the gels becoming more robust. The water uptake by the gels were low, L1 showed highest water uptake of 13% (2 h) and L4 showed least water uptake of 4.6% (2 h)