Tinosporacordifolia is a plant that has been found to be a rich source of bioactive compounds that can be used to synthesize copper nanoparticles (CuNPs). Spectrophotometric analysis confirms the presence of these nanoparticles, and their size and structure have been confirmed using energy dispersive x-ray (EDX), Fourier-transmission infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The study found that these CuNPs were able to control the growth of a specific bacterial species, Klebsiella pneumoniae, at a minimum concentration of 125 μg, while other bacteria such as Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were not affected at doses as high as 1000 μg. This suggests that these CuNPs have selective antibacterial activity and could be used as a safer alternative for future nanodrugs.