One of the areas of the pharmaceutical sector that is now expanding at a rapid rate is colon targeting, which makes therapeutic agents for specific medication delivery regardless of the challenges. Patients may respond better to medicine administration that specifically targets the colon. In pharmaceutical formulations, several polysaccharides are utilised. In the development of colon-focused drug delivery systems, nano-polysaccharide materials have assumed a prominent role. Thus, pH-dependent systems, microflora-dependent systems, multi-matrix technology systems, and drug delivery systems combining nanoparticles and osmotic pressure control have all been reviewed. Additionally, this study critically analysed and attempted to determine the significance of nano-Polysaccharides in the gradual development of colon targeting techniques.