Anemia is a common health issue among adolescent girls, particularly in underdeveloped areas like the province of Aceh. This stdy aims to investigate the impact of iron supplementation on the increase of hemoglobin levels in female students from Aceh province. This study employs an experimental design with a randomized clinical trial (RCT) in the city of Banda Aceh and Aceh Besar district. The research subjects consisted of 240 female high school students diagnosed with anemia. The data analysis used in this study is univariate and bivariate analysis (dependent t-test). The results demonstrate that the intervention group experienced a significant increase in their average hemoglobin levels after taking iron supplements, while there was no significant change observed in the control group. The independent t-test results also reveal a statistically significant difference in the average hemoglobin levels between the intervention and control groups (p < 0.05). This study highlights that iron supplementation can effectively enhance hemoglobin levels in female students from Aceh province.