Normal labor is a significant event in a woman's life with deep physical and psychological and emotional effects on the woman. This is the time of great hope, happy anticipation; labor can also be a period of fear, suffering and even death. Complications of pregnancy and labor are often the leading causes of maternal mortality and morbidity especially in the developing countries. Aim: This study aimed to evaluate the effect of instructional guidelines for maternity nurses’ performance regarding management of normal labor stages. Design: A quasi-experimental design (one group pre-posttest) was used in the study. Setting: The study was conducted at the obstetric and gynecological department in El Nabawi El Mohandas (public hospital in Fayoum) and Gamal Abdi El Nasser (Central Hospital Snores) at Fayoum city. Sample: A convenience sample was utilized in this study which included all maternity nurses (50). Tools: Two tools were used in this study: first tool A Structured interviewing questionnaire. Second Tool: Observational checklist regarding management of different stages of normal labor. Results: maternity nurses' knowledge &practice was improved after the implementation of instructional guidelines regarding management of normal labor stages, highly statistically significant difference between the level of studied nurse’s performance pre and post regarding management of normal labor stages. Conclusion: instructional guidelines have positive effect on nurse’s performance regarding management of normal labor stages as the levels of maternity nurses’ knowledge & practices regarding the management of normal labor stages was improved after the implementation of instructional guidelines regarding management of normal labor stages. Recommendations: Development of educational sessions regarding management of normal labor to provide maternity nurses update in knowledge and practices.