Background: Gold nanoparticles[AuNPs] were highly used recently in many therapeutic approaches and medical applications, a few is known about the effects of those nanoparticles on testis histology in animal model. Materials and methods: Thirty five healthy male adult albino rat Rattus norvegicus aged 10-12 week old with 200-230 body weight were used in the current study. Rats were randomly grouped into three groups: first group given distilled water, second group given 40mg/kg body weight of gold nanoparticles, third group given 80 mg/kg body weight of gold nanoparticles; for all the administration done orally for 60 days. After the 24 of administration animals were sacrificed and their blood was blood was withdrawed, serum obtained for the doing of hormonal assay by using ELIZA kits provided from Elabscience USA. For evaluation the level of Leutinizing hormone, Follicle Stimulating hormone, Testosteron and Estradiol. Results: Results were explained that low doses of gold nanoparticles for 30 days have less significant than the exposure for 60 days which were indicated that there was a decrease in the levels of (T,LH, FSH.)and increased Estradol hormone causing hyper aromatase syndrome , and there were an increase in ROS levels as a result of oxidative stress. Conclusion: Our present study indicated that a dose of 80mg\kg of body weight of gold nanoparticles could cause a deleterious toxic effects on the testicular structure more prevalently than the dose of 40 mg\kg of body weight,referring that low doses could cause less toxic effects and dose related toxicity.