In the present study the contents of Cu, Mn and Zn were investigated in the grains of winter wheat (Triticum aestivum L.). Plant samples were collected of the year 2004 when the weather conditions were very humid and wet. Samples were harvested and collected from five experimental stations of the Hungarian national long-term fertilization trials. These stations are as follows: Bicsérd, Iregszemcse, Karcag, Nagyhörcsök and Putnok. These experimental fields have different types of soil and climatic conditions. The elements content of samples were measured using inductively coupled plasma optical emission spectrometer (ICP-OES) and inductively coupled mass spectrometer (ICP-MS) followed by digestion with HNO3-H2O2 solution. Data analysis was done using SPSS for Windows 13.0 Software package. All data were subjected to ANOVA method, but after detection of significant differences (P<0,05) data were subjected to Duncan’s test to allow separation of means. The results of studies proved that the different regions caused significant (P <0.01) difference in the element content of winter wheat samples.