Novel 4-substituted pyran-2-one derivatives were synthesized by condensation of 6-methylpyran-2-ones and corresponding 2-aminobenzothiazoles. Condensation of 4-chloro-6-methyl-2H-pyran-2-one 2a and 2-aminobenzothiazoles 3(a-c) afforded corresponding 4-(2-benzothiazolylamino)-6-methyl-2H-pyran-2-one 4a, 4-(4-methyl-2-benzothiazolylamino)-6-methyl-2H-pyran-2-one 4b and 4-(5,6-dimethyl-2-benzothiazolylamino)-6-methyl-2H-pyran-2-one 4c. By condensation of 4-chloro-3-nitro-6-methyl-2H-pyran-2-one 2b and 2-aminobenzothiazoles 3(a-d), 4-(2-benzothiazolylamino)-3-nitro-6-methyl-2H[1]-pyran-2-one 4d and 4-(6-ethoxy-2-benzothiazolylamino)-3-nitro-6-methyl-2H[1]-pyran-2-one 4e were synthesized. The synthesized products were characterized on the basis of IR, 1H-NMR and 13C-NMR spectra. Compounds 4a-4e were screened for their antibacterial activity against S. Aureus, E. Coli and Klebsiella. Their antibacterial activity is examined by measuring the zones of inhibition around the disks impregnated with the corresponding product solutions in N,N-DMF concentration 2 mg mL-1, 4 mg mL-1 and 6 mg mL-1 and results are reported.