Chemicals make up the majority of microbiological growth inhibitors which are toxic and chemical solvents are bad for human health. Copper, silver, and gold metallic nanoparticles have several uses in biotechnology and medicinal research. Potent antifungal, antibacterial, anti-inflammatory, and anti-proliferative drugs are copper nanoparticles (CuNPs). In this study, nutmeg, a seed from the Myristica fragrans plant, was used to biogenically manufacture copper nanoparticles. The production of Myristica fragrans CuNPs involved dissolving copper sulphate in the extract. FTIR, EDX, and SEM were used to describe them. These CuNPs are between 20 μm and 200 nm in size, according to SEM tests. Copper and oxygen were detected, and the metal's oxidation state was validated by EDAX analysis. The nanoparticles' distinctive functional groups were discovered using FTIR spectroscopy. The anti-fungal activity of Myristica fragrans seed extract was assessed. The extract from the seeds of Myristica fragrans and caffeic acid ,a bioactive component exhibited the strongest antifungal activity. According to these findings, antifungal CuNPs Phyto-formulated with nutmeg extracts may one day be employed to treat microbial diseases