Synthesis of any organic compounds are depending on each and every atom of the compounds and all substitutes are linked with the rings to exhibit novel properties with the involvement of minimum atoms for the organic synthesis using carbon and hydrogen molecule. To investigate the novel flavonoid compounds of quercetin derivatives were synthesized using ethyl and methyl alkyl substituents of saturated groups. All the synthesized derivatives of quercetin separated by chromatography and characterized by spectroscopy such as infrared (IR) and fourier transmission infrared (FTIR), liquid chromatography and mass spectroscopy (LCMS, 1H and 13C nuclear magnetic resonance (NMR) to elucidate the compounds. The synthesis of quercetin derivatives like 4'O-methyl quercetin, 4'O-sulphate quercetin and 4'O-glucose quercetin obtained as compared to standard compounds. Quercetin compounds were reacted with a multi fold molar excess of ethyl and methyl mixtures, and the obtained products were analyzed and confirmed by HPLC and LCMS. All the three disulfates were identified, and structural inferences were drawn by 1H and 13C NMR spectrometry of HPLC peak isolates.