The present research work was conducted in mulberry growing soil samples collected from twelve different villages of three different mandals (Hindupur, Parigi and Lepakshi) of Anantapur district, Andhra Pradesh during the year 2020. The standard methods were employed for the estimation of various physico-chemical parameters such as pH, organic carbon, electric conductivity, nitrogen, phosphorus and potassium. It is evident from the present study findings that the mean values of pH were ranged from 6.43±0.57 to 6.92±0.40. Almost all soil samples were acidic in condition. The mean values of Electric conductivity were ranged from 0.04±0.005 to 0.06±0.014 and the reported EC values were sufficient in condition. Similarly the organic carbon content ranged from 0.50±0.20 to 0.61±0.09 and the recorded OC values were sufficient in condition. Likewise the available nitrogen ranged from 184.36±11.96 to 203.93±33.91 and the recorded N values were sufficient in condition. The mean values of phosphorus ranged from 113.39±11.76 to 119.12±23.14 and the reported values were high in condition. Finally the mean values of potassium ranged from 161.63±32.61 to 200.67±41.30 and the reported values were sufficient in condition.