Background: Acintobacter baumannii (A. baumannii) associated with hospital acquired sepsis in pediatric patients represent health care problem. The multidrug resistance of A. baumanni is a risk factor associated with this organism. Objective: The present study aims were to i. study the presence of virulence factors of A. baumannii isolated from bacteremia from children in ICUs, ii. Study antibiotics resistance of isolated A. baumannii regarding ESBL and carbapenemase, iii. Study the association between the virulence factors, extended-spectrum-β-lactamase (ESBL) and crabapenemase activity. Method: The study was a retrograde cross-sectional study that included one hundred children with hospital acquired bacteremia admitted to intensive care units. The isolates were subjected to polymerase chain reaction (PCR) to detect virulence genes bap (biofilm-associated protein), phospholipase (plcN), elastase (lasB), biofilm-associated protein (bap) and outer membrane protein A (Omp) genes. The sensitivity to antibiotics was determined by discs diffusion method, ESBL and crabapenemase activity was determined by double discs diffusion and combined disc diffusion method respectively. Results: The isolated A. baumanni were ESBL producers (40%) and carbapenemase producers (96%). The isolates of Acintobacter baumannii had high frequency of bap and OmpA genes (95%, 70% respectively) and lower frequency of picN and laspB genes (26%, 8% respectively). The A. baumannii isolates with ESBL activity had significant association with OmpA genes (90%, P=0.001) and 57.5% of the isolates had significant association with picN gene (P=0.001). There was significant association between MDR of A. baumannii and the presence of OmpA gene and plcN gene (P=0.048, P=0.001 respectively. The present study highlight the presence of virulence genes bap, OmpA, picN and laspB in clinical isolates of A. baumannii isolated from pediatric patients with sepsis. Almost all the isolates had bap gene and more than half isolates have OmpA gene. The ESBL resistance and multi- drug resistance of A. baumannii was significantly associated with the presence of OmpA and picN genes.