Background: Coronavirus disease (COVID-19) is caused by the Severe acute respiratory syndrome coronavirus 2 (SARS-COV2) which causes mainly pneumonia and other clinical signs due to inflammation and cytokine storm, which leads to rise in certain inflammatory markers. And studying these laboratory parameters with disease causation and progression would be beneficial for clinicians to predict prognosis of the disease. Aim and objectives: To study the relation of laboratory parameters (serum Ferritin, serum D-dimer, serum LDH, CRP, ESR) of Covid-19 positive patients with severity and outcome of Corona virus disease (Covid-19). Material and methods: This was a single centre, hospital-based prospective, observational study, conducted on patients admitted to COVID ward and intensive care unit of the tertiary care Hospital. This study was conducted over a period of 18 months from February 2021 to August 2022.A total 100 patients were included in this study satisfying the Inclusion criteria. All enrolled patients were tested for serum ferritin, serum D-dimer, serum LDH, CRP, ESR. The above data was collected and complied in Microsoft excel. Results of quantitative data measurement were presented on mean +/- SD (min-max) and qualitative data measurements were presented in percentage and proportions (%). Comparison of qualitative variable was analysed by chi-square test. A p value of <0.05 was considered statistically significant. Data analysis was done using open epi version 2.3.1. Results: A total of 100 patients were enrolled in the study, 67% were males and 33% were females, predominated by the male gender with a male to female ratio of 2:1. It was observed that there was association between positive inflammatory markers (i.e. D-Dimer, Ferritin, LDH, ESR, CRP) with the severity of COVID-19 disease and was statistically significant . Conclusion: Present study concluded that the inflammatory markers like ESR, C-reactive protein, Lactate dehydrogenase, serum Ferritin and D-Dimer were elevated in severe cases and had adverse outcomes. Thus, these inflammatory markers can be used to risk stratify prognosis of COVID-19.