Benzene sulfonyl hydrazide (1) undergoes facile condensation with aromatic aldehydes 2(a-h) afforded the corresponding N-arylidene benzene sulfonyl hydrazides (3a-h) in good yield. Cyclo condensation of these hydroxides (3a-h) with chloro acetyl chloride yielded N-(3-chloro-2-oxo-4-arylazetidin-1-yl) benzene sulfonamide (4a-h). The reaction of Sulfapyridine with N-(3-chloro-2-oxo-4-aryl azetidin-1-yl) benzene sulfonamide (4a-h) produce. 4-((2-aryl-4-oxo-1- (phenylsulfonamido)azetidin-3-yl) amino)-N-(pyridin-2-yl) benzenesulfonamide (5a-h). The structure of these compounds were established on the basis of analytical and spectral data. All the produce compounds (4a-h) and (5a-h) were evaluated for their antibacterial and antifungal activities by agar cup method.