Developed an accurate, precise and reproducible ultra performance liquid chromatographic method (UPLC)for simultaneous estimation of Lopinavir and Ritonavir in bulk and tablet dosage forms. Chromatographic separations of the drugs were achieved on a Gemini C18 (4.6×50mm, 5.0 µm) using Isocratic elution of Water: methanol (30:70) pH 2.8 adjusted by using ortho phosphoric acid at flow rate of 0.5 ml/min. UV (Ultra Violet) detection was performed at 215 nm. The retention time obtained for the lopinavir was 0.502min and for the ritonavir was 1.583min. The calibration curves were linear over the range of 0-100 μg/mL and 0-25 μg/mL for lopinavir and ritonavir respectively. The method is validated as per ICH guidelines by determining its specificity, accuracy, precision, linearity and range, ruggedness, robustness and system suitability.