Simple, sensitive and reproducible spectrophotometric method was developed for estimation of oxymetazoline hydrochloride via charge transfer complex formation reaction. The method is based on the reaction of oxymetazoline as n-donor with DDQ reagent as π-acceptor in basic aqueous medium of pH 9.79. The maximum absorption is 421 nm. Beer's law was obeyed in the range 0.4-4.0 μg mL-1 with molar absorptivity 2.5 x 104 L mol-1 cm-1. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.0093 and 0.028 μg mL-1 respectively. The stability constant has been determined and the mechanism of the reactions was proposed. The proposed method was applied to determine the oxymetazoline hydrochloride in dosage form as drop, and the results were statistically compared with official BP method.