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ISSN 2063-5346
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SIMULTANEOUS ESTIMATION OF CLONIDINE AND HYDROCHLOROTHIAZIDE IN TABLET DOSAGE FORM BY VALIDATED STABILITY INDICATING RP-HPLC METHOD

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Purushottam Prajapati , M.S. Ranawat
» doi: 10.48047/ecb/2023.12.si5.093

Abstract

Objective: To develop and validate a simple, efficient and cost-effective stability indicating RP-HPLC method for simultaneous estimation of Clonidine and Hydrochlorothiazide in Tablet dosage form. Methods: Phosphate buffer (ph6.0)-Methanol and Triethylamine (70:30:0.1) used as mobile phase and stationary phase (250*4.6mm C18, Hypersil BDS) column, wavelength was selected 226 nm, Flow rate 1.0 ml/ min, injection volume 20 µl and column oven temperature 25°C. Prepared Standard solution and sample solution at working concentration, used Mobile phase as diluent

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