Background:Acyclovir is an antiviral drug, also known as 9-[(2-hydroxyethoxy) methyl] guanine, is a purine nucleoside analogue that prevents the replication of strains 1 and 2 of the herpes simplex virusas well as the viricella zoster virus.NicardipineHClis a dihydropyridine. It possesses a strong calcium channel blocker and a vasodilator effect. Objectives:The current study aims to design and evaluate an easy simultaneous equation for UV spectroscopy approach for estimating both Acyclovir,NicardipineHCl in a mixture. Methods: Result:Result of this experiment gives value of the unknown concentration, explains linearity and range of both the drugs like for Acyclovir,y = 0.114x + 0.040,R2 = 0.995 and for NicardipineHCl,y = 0.122x + 0.015, R2= 0.998. The range was observed by the drug is 2 -10μg/mL having λmax at 255 nm for acyclovir and 235 nm for nicardipineHCl. The Accuracy was found to be 99-100% and precision was less than 2 giving the affirmation that method was precise and drug sample were pure. LOD and LOQ are also mention with their method of detection.