The endemic viviparous fish Dermogenys orientalis is locally known as the "Anculung fish" and belongs to the family Zenarchopteridae. There is presently no data available regarding the maturation aspect of Anculung fish. This research examines sexuality, gonadal maturity, size at first maturity, and gonado somatic index as maturation features. From October 2021 to March 2022, the sampling period lasted six months. This research collected 366 fish samples, including 126 males and 240 females. Anculung fish exhibit size dimorphism, with males being smaller than females. Based on their sexuality, andropodium is present in males but absent in females. The acquisition of distinct maturation levels differs. Males are also smaller than females when mature. The total length at first maturity for males is 48.3 mm, and for females it is 53.6 mm. Females have higher GSI values than males. According to the analysis, Anculung fish are capable of reproducing every month