Aim & Objectives: To evaluate the analgesic effect of Venlafaxine in experimental Albino mice. Materials and Methods: The study was conducted after Institutional Animal Ethics Committee approval (IAEC). Animals were divided into four groups. Group 1 (Control group) received Distilled water (2ml/kg), Group 2 (Diclofenac group) received Diclofenac (10mg/kg), Group 3 (Venlafaxine 50mg/kg group) received Venlafaxine (50mg/kg) and Group 4 (Venlafaxine 100mg/kg group) received Venlafaxine (100mg/kg). Eddy’s Hot plate method was used to investigate analgesic activity of Venlafaxine. Results: The present study clearly shows that Venlafaxine 100mg/kg has significant analgesic activity at 30 and 60 minutes testing time intervals (p < 0.05) when compared to control and its activity was comparable to Diclofenac at 30, 60 and 90 minutes testing time intervals (p > 0.05). Conclusion: Venlafaxine 100mg/kg has significant analgesic potential in Eddy’s Hot Plate method.