Life-threatening infections can be caused by microorganisms such bacteria, viruses, protozoa, fungus, and a few different types of worms. Antibacterial or antibiotic medications are used to stop or slow the growth of bacteria. Yet, these microorganisms have developed antibiotic resistance, which makes the situation worse. Thus, using medicinal plants is a safe alternative. Often referred to as "dikamali," Gardenia resinifera Roth. is an anthelmintic and a remedy for children's toothaches and teething issues. This study further evaluates the anti microbial activity of Gardenia resinifera. The plant material was collected & sujected to maceration followed by various qualitative & quantitative tests. Results showed that the pet ether extract was found to be enriched with diterpenes, phenol & carbohydrate while hydroalcoholic extract contain flavonoid, diterpene, phenol, proteins, carbohydrate & saponins. Total phenol & flavonoid content in hydroalcoholic extract was found to be 0.65 mg/ 100 mg and 0.72 mg/ 100 mg respectively. Maximum antimicrobial activity of extract was found to be against E.coli wiith the zone of inhibition 20 ± 0.47mm. While for Bacilus subtilis&C. albicans it was observed to be 15 ± 0mm & 19 ± 0.47mm respectively. From the results it can be concluded that Gardenia resinifera have convincing anti microbial activity against screened microorganisms.