Biotechnology researchers are particularly interested in medicinal plants since they are used by the majority of the drug industry to produce pharmaceutical chemicals. Crude plant extracts which are made up of a complex combination of several phytochemical ingredients (plant secondary metabolites), are the primary ingredient in the majority of herbal medicines and their derivative products. These components' chemical characteristics vary greatly between species. The present investigation involves three different solvents of extract the leaves from Connarus wightii, the methanol extract of Connarus wightii shows the antibacterial activities in both Gram-positive (Staphylococcus aureus) and Gram-negative (Aeromonashydrophila) bacteria strain compared to other solvent extracts. In this studies the three different solvents of extract show high activity in 500 μg/ml concentration, the methanol, chloroform, and ethanol extracts of Connarus wightii exhibit greater inhibitory action against Staphylococcus aureus than Aeromonas hydrophila.Compare all of the extracts to the standard drug, fluconazole; the extracts had no effect against Aspergillus niger and very moderate efficacy against Candida albicans. As the concentration of extracts (μg/ml) the antifungal activity of the extracts also increased linearly. The results showed that when compared to typical medications, fungal activity from Candida albicans performed better than that of Aspergillus niger. GC-MS method used for the analysis of the obtained extracts can be an interesting tool for testing the amount of some active principles in herbs used in cosmetic, drugs, pharmaceutical.By using spectrophotometer was used to calculate the protein extracts from the C. wightii leaf sample's absorbance at 600 nm. Maximum protein content of 86.03 ± 0.008μg/ml The SDS-PAGE electrophoresis was carried out to estimate the molecular weight protein against the protein standard.The most abundant protein from powder and fresh leaves band occurs at 63kDa