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ISSN 2063-5346
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Screening, identification and characterization of Polyhydroxybutyrate producing bacteria from garden soil

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1Mahitha S, 2 Sasi Premila J.M, 3Abiya Chelliah D
» doi: 10.48047/ecb/2023.12.5.294

Abstract

Plastic pollution should be resolved as it affects air, land and water. The favourable alternative for plastics would be Polyhydroxybutyrate (PHB) from bacterial origin, which are biodegradable and biocompatible biopolymers. Focus on the PHB producing bacteria is done by collecting garden soil sample. Five colonies of Sudan black blue positive isolates were chosen, extracted, and PHB was produced. One of the strains (SM1) - a potent producer as confirmed by crotonic acid assay, was further subjected to large scale production. The PHB thus produced was analysed using Fourier Transform Infra-Red spectroscopy (FTIR) and Gas Chromatography Mass Spectrometry (GCMS) to confirm the presence of functional groups. Scanning electron microscopy helps in picturised the microstructure and surface morphology of PHB. DNA was isolated from the strain SM1, and the gene for 16S rRNA has been sequenced and submitted in GENBANK, (Accession No: MZ363886). The organism was found to be Bacillus cereus as predicted by 16S rRNA and BLAST (Basic Local Alignment Search tool).

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