Agriculture workers are at higher risk of exposure to organophosphorus (OPs) pesticides. In this regard, farmers are at high risk as they are highly and more frequently exposed to OPs without knowing the handling OPs safely. Creating awareness about the hazards of exposure to OPs can result in a drastic reduction in morbidity and mortality rate. This is an educational interventional study carried out for six months among agriculture workers and farmers of selected villages across the state of Tamil Nadu in southern India. All the participants were administered with pre and post-tests using a structured questionnaire. After the pre-test educational session was conducted using informational leaflets, and audiovisuals. Post-test was conducted after 2 weeks of Pre-test. Overall awareness among the agriculture workers and farmers about OPs in terms of knowledge, safety-handling, and first aid was found inadequate during the pretest but was significantly improved after the educational sessions during the post-test which revealed and emphasized the role of the pharmacist in public health education.