Immunoglobulin E (IgE) occurs in extremely low amounts in blood and tissues but is the key immunoglobulin in allergy. The quantification of allergen-specific IgE levels (sIgE) in serum and body fluids is important because sIgE concentrations are useful to define clinical phenotypes of allergy and to predict the intensity of allergic reactions. Aim & objectives: The purpose of this study was to analyse the role of IgE ELISA for diagnosis of allergy in patients with respiratory and skin allergies. Material and Methods: Study comprises of 147 patients, with clinically suspected allergic reactions. The serum samples was taken and subjected to ELISA. Results: Among the 147 patients, who fulfilled the inclusion criteria were screened for IgE ELISA testing, 114 (77.5%) were found to have raise in IgE levels. The raise in IgE levels was predominantly seen in females (94) as compared to male cases (53). Conclusion: The presence of specific IgE is an indicator of sensitization to allergens. Identification of specific allergens in allergic patients enhances management through education, allergen avoidance, and immunotherapy, each of which can improve clinical outcomes.