The naturally occurring imidazole core scaffold have shown interesting biological as well as physiochemical properties and consequently they have found several chemical, optical, pharmaceutical, and other useful biological applications. Imidazole moiety contains three carbon atoms, and two nitrogen with electronic-rich characteristics that are responsible for readily binding with a variety of enzymes, proteins, and receptors compared to the other heterocyclic rings. In this review, we have summarized all the pharmacological, biological and chemical applications of imidazole derivatives. Herein, we provide a thorough overview of the current research status of five membred imidazole-based compounds with a wide variety of biological activities including, anti-bacterial, antifungal, antiprotozoans, anti-parasitic, anti-cancer, anti-inhibitory, and anti-inflammatory. In this review, we have also discussed anti-oxidant, anti-corrosion behavior and luminescence properties of the imidazole derivatives.