Bacteria predominance in periodontitis, antimicrobial potential of Uncaria gambir and catechol intended present investigation to compare the antimicrobial potential of hydrazide derivative of catechol (HDC) and hydroalcoholic extract of Uncaria gambir leaves (HEUGL) against periodontitis associated bacteria (PAB). Study involved synthesis of HDC and preparation of HEUGL. Synthesized compound was characterized using ATR-IR, 1H-NMR and Mass spectrometric data. Both HDC and HEUGL were further tested for their antibacterial potential against PAB such as Escherichia coli and Staphylococcus aureus. Both HDC and HEUGL exhibited high antibacterial potential against PAB, however HDC exhibited much higher antibacterial potential. Present study concludes that HDC possess high antibacterial potential against PAB and recommends that HEUGL should be further evaluated for its preclinical significance