Mothers are directly responsible for the dental health of their children and can play an important role in preventing oral diseases in children. They clean teeth of their children, teach them proper hygiene and dietary habits, and organize professional dental care. Aim of the study: was to assess relation between Mothers' Awareness and oral health status of preschool children. Design: A descriptive cross-sectional design was utilized to conduct the current study. Setting: the study was conducted at governmental nursery schools (11nurseries) in Awlad Sakr city at Al Sharkia Governorate. Sample: The current study enrolled 400 preschooler mothers and their children. Tools: Three tools were used to collect study data include1) an Interview questionnaire composed of three parts: Socio-demographic characteristics, the dental history of child, Data related the mother during pregnancy and labour, 2) Mothers' awareness tool, 3) Observation checklist for oral health assessment. Results: Study results found that 75.5% of the studied mothers had satisfactory total awareness regarding oral health and 36.7% of preschooler had poor or un healthy oral condition. Moreover there were statistically significant relation between mother’s awareness and oral health status. Conclusion: Statistically significant negative correlation was found between mothers’ awareness and child oral health status this mean the lower mothers awareness lead to the higher unhealthy oral status among preschoolers. Recommendations: Continuing awareness programs about oral health of preschool children is a useful in preventing and early detection of oral health diseases.