In recent years, solid state technology is most challenging and it focus towards the proficient electrochemical storage system consuming high conductivity and energy power with non-toxic and reasonable cost. This research work is primarily, concerned with the study of biopolymer electrolyte membrane that exhibit a standard ionic conductivity range. Research has been done to create a environmental acceptable biopolymer electrolyte, Chitosan (CT) complex with Ammonium Nitrate (NH4NO3) salt by solution-casting method. Ammonium nitrate with two different compositions ranging of 0.5g and 0.7g has been added to rise the ionic conductivity of Chitosan based biopolymer film. The obtained electrolytes were characterized by XRD, FTIR, and EIS, CV, LSV study. Addition of salt enhances the amorphous nature of the membranes, as confirmed by XRD. The compositions of CT (1g) with (0.7 g) NH4NO3 exhibit an excellent degree of amorphous nature. FTIR analysis reveals the complex and vibrational formation between the salt and biopolymer. AC impedance investigation, the ionic conductivity value for CT (1g) with (0.7g) NH4NO3 has been determined to be 1.9×10-2 S/cm. Dielectric permittivity (ε*) has been used to study the dielectric behavior of electrolyte. As the NH4NO3 content rise, the ε’ and ε” increased as well and proving that they are ionic conductors. The maximum ionic conductivity membrane’s electrochemical range of 1.16V has been determined by the application of Linear Sweep Voltammetry (LSV). The final output results suggest that the highest conducting electrolyte film, function well as a proton conductor in electrochemical system.