The recent advent of nanoscience and nanotechnology as a cutting edge science has created oppoertunities for novel and creative uses as herbal nanomedicine. In this present research, high energy Ball milling was used to produce the superfine nanoparticles from shade-dried Aegle marmelos leaves (bael). The SEM analysis shows highly agglomeration in the milled sample. The particle size was evaluated using TEM analysis and found to be 19.89 nm. The Zeta potential found to increase with milling hour from -15.56 mV to -22.57 mV, showing the stability of material increases with milling hours. Despite the nanoparticles' size changes as a result of milling time, and crystallinity of prepared bael nanopowder remained unchanged, which were confirmed by XRD measurement