Nano-sized reduced graphene oxide (RGO) shows potential for biomedical uses such as photothermal therapy of cancer. It is discovered the novel property of RGO nanoparticles, which release a significant amount of heat by the stimulation with near infrared leaser. This phenomenon supports the hypothesis thermal cytotoxicity of cancer cells by the induced leaser stimulated heat. The synthesis of RGO from commercial graphite procurer was carried out using improved hummer method followed by green reduction with ascorbic acid. Morphological characterization carried out via scanning electron microscopy (SEM) and transmission electron microscopy (TEM) demonstrated the successful exfoliation of graphite procurer to RGO. Furthermore, Raman spectrum of RGO exhibited G band at 1582 cm−1 (typical also for graphite), and broad D band (characteristic for RGO) appears at 1350 cm−1 ; this characteristic peak confirmed the successful formation of RGO. Tumor bearing mice were divided into four groups three served as positive and negative control groups the treated group injected by RGO and irradiated by 980nm laser. The assessment of tumor response showed marked response of tumor to hyperthermia produced by RGO stimulated heat induced by near infrared laser