Rosuvastatin calcium, an antihyperlipidemic agent with poor water solubility which comes under BCS Class II with 20% bioavailability, was chosen for the study. The present study focuses on Preparation, Evaluation and Optimization of Nanosuspension of Poorly Water-Soluble Rosuvastatin Calcium. Rosuvastatin Calcium Nanosuspension was prepared used Solvent-Anti-Solvent Precipitation method. Experimental Design was obtained using Design Expert Software. 10 Batches of Rosuvastatin Calcium Nanosuspension was prepared. Interaction between Independent factor and dependent factor was studied. Optimization was carried out and Batch B9 was selected as the Optimized Batch. Batch B9 was Prepared using 1:10 Ratio of Solvent-Anti-Solvent, 6 % Concentration of Stabilizer was used and it was sonicated for 10 minutes. The Results of the Formulation B9 was also satisfying. The Particle Size of the Optimized batch was found to be 351.64 nm, Zeta Potential was -20.21 mV and % Drug Release was 88.52 % in 60 minutes. Stability studies of B9 was carried out and no significant growth in Particle Size was found which indicates Good Stability. Comparison of in-vitro % Drug Release of Pure Drug and Optimized Batch of Rosuvastatin Calcium Nanosuspension was carried. Optimized Batch B9 showed 88.52% Drug Release in 60 min while Pure Drug Showed 26.29% drug Release in 60 min.