Background:Elemental analysis, metal content, chloride content, molar conductance, FT-IR spectra, 1H-NMR, UV-Vis spectra, magnetic susceptibility, and mass spectra have all been used to analyze the ligand and its metal complexes, Mn(II), Ni(II), and Cu(II), Around the world, xanthine alkaloids are frequently used as pharmacological agents; they occur naturally in beans cacao and beverages popular such as coffeeand tea Objective:to preparation of a new Schiff base ligand (HL1) from the condensation of 1-3-dimethyl-1H-purine (2,3-((3H,7H) dione and 4-aminophenol). Materials and methods:Antioxidant efficiencies from compounds had been estimated versus 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical as well then they have been a subject of comparison with Gallic acid that employed as phenolic reference in order to make a study of the antioxidant activity. Results:The outcomes illustrate that the compounds display exceptional root scavenging efficacies. Conclusion:Expression profile of miRNA-125b can be used as novel markers for AS.