Investigating the phytochemical components of an anti-diabetic polyherbal composition was the goal of the current study. Azadirachta indica leaves, Ocimum sanctum leaves, Boerhaavia diffusa flowers, and Aegle marmelos fruits were used to make the mixture. Following accepted techniques, the composition's phytochemical qualities were assessed. The composition showed the presence of active phytoconstituents like Quercetin and Ferulic acid as well as carbohydrates, amino acids, proteins, phenols, and flavonoids. At 254 nm, the HPTLC fingerprint analysis identified 18 bioactive substances. The research supports the formulation's presence of numerous phytoconstituents, making continued development of it a viable option