Aim: Although the safety of the polyherbal ayurvedic remedy Gokshuradi Churna (GC) is unknown, it is useful for inflammatory conditions like arthritis and other joint problems. The examination of heavy metals, the detection of aflatoxin, the presence of pesticide residue, and microbiological counts have not yet been assessed. The parameters above were assessed in the current investigation. The objective of the current study was to assess the safety criteria (heavy metal, aflatoxin, pesticide residue, and microbiological profile) of the formulations GC-I, GC-II, and GC-Marketed Formulation (MF). GC-I and II were developed internally. Materials and Methods: According to AYUSH [Ayurveda, yoga, unani, siddha, naturopathy] Pharmacopoeial laboratory for Indian medicine (PLIM) guidelines, the formulations were evaluated for its safety parameters at Indian Institute of Chennai (IIT), Chennai and Tamilnadu Test Solutions Private Limited, Chennai was used for testing heavy metals and aflatoxins were tested using thin layer chromatography (TLC). The Pesticide residues content was estimated by Gas Chromatography while microbial count by pour plate method. Results: Results: According to AYUSH Pharmacopoeial Laboratory for Indian Medicine guidelines, the presence of the heavy metals mercury, arsenic, lead, and cadmium was within the recommended limit. However, aflatoxins, pesticide residues, and microbes were not found in the samples, indicating that the formulation Gokshuradi Churna was toxic-free. Conclusion: According to the PLIM criteria of AYUSH, GC revealed heavy metal concentration below the allowed limit. Microbes and particular pathogens were not present in the GC samples, and neither were aflatoxins or pesticide residues. Since the formulations were safe for therapeutic use, the current study provides that assurance.