.

ISSN 2063-5346
For urgent queries please contact : +918130348310

PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITIES OF VARIOUS MEDICINAL PLANT EXTRACTS

Main Article Content

Mukti A Jadav, Dr. Gaurang B Shah
» doi: 10.53555/ecb/2022.11.01.30

Abstract

Kigelia pinnata, Cichorium intybus and Cedrous deodara are traditionally used medicinal plants against several diseases. This study aims to undergo qualitative, quantitative and antioxidant properties of several extracts from these plants using in-vitro assay. Qualitative study of extracts thin layer chromatography (TLC) was carried out. Quantitative analysis to determine total content of phenolic, flavonoid, iridoid and terpenoid using specific chemical reagents. DPPH (Free radical diphenylpicrylhydrazyl), Phosphomolybdate (Total antioxidant capacity) and reducing power assay were used to measured antioxidant potential to each extracts. TLC revealed presence of phenylpropanoids, naphthaquinones, flavonoids, phenolics, terpenoids, volatile oils, organic acids in different extracts. High phenolic content was found in methanolic extract of C. intybus and K. pinnata fruit root (11.97 ± 0.37 and 11.11 ± 0.37 mg/g). Flavonoids were higher in both ethanolic and methanolic extracts of C. intybus root (6.12 ± 0.32 and 5.80 ± 0.96 mg/g). Terpenoid content was higher in ethanolic extract of C. deodara wood oil (5.714 ± 0.71 mg/g). Iridoids were found only in methanolic extracts of K. pinnata. With the exception of methanolic extract, all extracts had high free radical scavenging properties, but they were all noticeably less effective than ascorbic acid. Reducing power activity was significantly lesser in all extracts as compared to Quercetin. The extracts are rich in various important phytochemicals and seem to have good antioxidant potential but need to be assessed using biological assays.

Article Details