Cestrum nocturnum Linn. belongs to Solanaceae. It is commonly known as Raat rani, lady of night or night Jessamine have great medicinal value. The plant was extracted using conventional extraction as well as microwave-assisted extraction. The phytochemicals such as carbohydrate, proteins, amino acid, glycoside, phenolic compounds and tannins have been qualitatively determined. The physicochemical properties such as relative density, viscosity, surface tension and refractive index were determined. All features of microwave assisted extract were found to be higher than aqueous extract. UV-Vis and IR data were evaluated. The extracts were screened for biological activity and aqueous extract is found to be active against E. Coli, B. Subtilis, S. Typhi, S. Aureus, tuberculosis and malaria