The plant extracts were found to be the best source of natural antioxidants in comparison to synthetic ones, as their nature is non-toxic, medicinal properties, easy isolation process and mechanism of action. Herein, it is intended to investigate the in vitro antioxidant and in vitro hepatoprotective feature of leaf extract of Biophytum umbraculum. Standard methods were used for the identification of flavonoids, alkaloids, carbohydrates, saponins, tannins, sterols, terpenoids, reducing sugars and amino acids. Further, through quantitative analysis, total flavonoid, phenolic, tannin components, antioxidant feature and total reducing power were analyzed. Additionally, the DPPH free radical scavenging activity was studied with respect to vitamin C as standard, nitric oxide radical scavenging activity, iron chelating assay and superoxide dismutase scavenging activity were also investigated. The Biophytum umbraculum extract was found to be an efficient antioxidant with an IC50 value of 576.7±25.2 µg/ml. MTT assay revealed the % cell viability on HepG2 cells as 90.75 (200 µg/mL of Biophytum umbraculum leaf extract alone) and 99.28 (200 µg/mL of Biophytum umbraculum leaf extract on cells induced with H2O2) whereas 38.05 % ROS inhibition activity was observed at 100 µg/mL of Biophytum umbraculum leaf extract in H2O2 induced HepG2 cells. A dose-dependent response was evident in SOD and NO assays. The antioxidant property of 70% hydro alcoholic leaves extract of Biophytum umbraculum was confirmed and attributed to the phenolic components. The same of other extracts with active chemical constituents could be explored further.