The need for antibiotic-tolorated bacteria, combined with the negative effects of antibiotics on human health, necessitates the pursuit of plant-derived antimicrobial compounds. Plants with ethnomedicinal uses can be investigated to reveal their efficacy for the discovery of novel secondary metabolites and herbal drugs. Citrus medica L. leaf is used against loose motion and skin diseases by the tribal communities of the Balangir district of Odisha. Therefore, the study was designed to test the presence of different secondary metabolites in different leaf extracts of Citrus medica L., such as n-Hexane and Ethanol as solvents using the Soxhlet apparatus and to screen its antibacterial test using the agar well diffusion method as well as to determine the minimum inhibitory conc. (MIC) using the serial dilution method. The results of the phytochemical screening showed that the plant's secondary metabolites were present abundantly in the methanol extract. The leaf extracts in different test solvents were observed to have broad-spectrum activity thereby showing their efficacy against both Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Shigella flexneri, and Salmonella phimurium). The ethanolic extract of Citrus medica L. showed maximum antimicrobial potential against Salmonella typhimurium , with a larger zone of inhibition (15.13 ± 0.14) and lower MIC value (25 mg/ml) as compared to the other two extracts against the four bacterial strains. Hence, further investigations could reveal that the leaf extracts of Citrus medica L. can be used as an effective natural antibacterial agent for the treatment of different bacterial diseases.