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ISSN 2063-5346
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Phytochemical Analysis and Evaluation of Wound Healing Properties of Cucurbita pepo Linn, Colocasia esculenta (L) Schott and Amaranthus graecizans Linn

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Sonia Singh Thakur, Manmohan Singh Jangdey, Khomendra Kumar Sarwa
» doi: 10.31838/ecb/2023.12.sa1.119

Abstract

Wound healing is a typical biological process. It involves four intricate steps: homeostasis/coagulation; inflammation, migration, and proliferation; reepithelialization; and restoration. A number of mediators, including platelets and cytokines, inflammatory cells, cellular and extracellular matrix, proteinases, growth factors, and inhibitors, have an impact on each stage of the healing process for wounds. In this study, the methanolic leaf extracts of Cucurbita pepo Linn (CP), Colocasia esculenta (L.) Schott (CE), and Amaranthus graecizans Linn (AG) were evaluated for their ability to promote wound healing. An established test technique that is documented in the literature was used to determine the qualitative analysis of different phytochemical elements. Rats of either sex were used to test an excision- and incision-based wound model. Five groups of six Wistar albino rats were created; group I (left untreated) was used as the negative control, group II was given 5% (w/w) povidone iodine ointment (Intadine USP) as the standard, group III received 5% (w/v) CP extract, group IV received 5% (w/v) CE extract, and group V received 5% (w/v) AG extract as the test group. Each therapy was administered once daily. The percentage wound contraction, epithelialization time, hydroxyproline content, and histoarchitecture studies in the excision wound model and tensile strength in the incision wound model were used to measure the wound healing effect. In both of the investigated animal models, extracts greatly aided wound healing activity. The methanolic extracts of the leaves of CP, CE, and AG contained alkaloids, terpenoids, flavonoids, carbohydrates, tannins, and saponins, according to a phytochemical screening. In CP-treated rat, there was a high rate of wound contraction, a reduction in the time needed for epithelialization, and an increase in the hydroxyproline level.

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