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ISSN 2063-5346
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Physiological and Biochemical Study of Modulation of Pituitary- Adrenal Axis, Pituitary-Gonadal Axis and Reproductive Efficiency Responses to Stress Induced by Exposure to Toluene in Rabbits

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Arwa H. AL-Saeed, Fouad Ziedan Hamza, Muna H. AL-Saeed
» doi: 10.31838/ecb/2023.12.sa1.290

Abstract

Toluene is a widely abused inhaled solvent. The current study has been designed to conclude whether toluene abuse affect the pituitary-adrenal axis(PAA), reproductive functions of both sex of rabbits. Five–month–old male and female rabbits were exposed to toluene vapor inhalation (8000ppm; 2h/day) daily for 30 days resulted in a dose-dependent salivation and lacrimation were observed during exposure periods and intensified with recurrent contact. Suppression of total body weight gain and food utilization were experiential in toluene group. Females were exposed from 30 days before mating until day 7 of gestation. The serum and ovary of the rabbits were collected; ELISA was applied to determine the sex hormone(Estradiol (E2) and Progestron (P4), Testosterone(T), corticosterone ) in serum; the pituitary, adrenal, ovary samples were stained with haematoxilyne and eosin and the number of follicles was calculated. The sex hormone of the low concentration group treated with tolune was significantly lower than that of the control group(P≤0.05);no significant difference was found in P4 and the number of follicles between different groups.

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